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KMID : 0984920100120010119
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Journal of Skin Barrier Research
2010 Volume.12 No. 1 p.119 ~ p.119
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Effect of Persimmon Leaf Extract on UVB-induced Response in Mice
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Jang Seon-Il
Cho Jung-Keun
Park Kwang-Hyun
Mok Ji-Ye
Park Ji-Min
Jeon In-Hwa
Kim Hyeon-Soo
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Abstract
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UVB light can activate inflammatory pathways, leading to skin damage. Persimmon leaf extract (PLE) has been raditionally used for acute and chronic diseases in Oriental Countries but no reported on the UVB protective effect. The purpose of this study was undertaken to investigate the protective effect of PLE on UVB-induced cytotoxicity in HaCaT cells and skin damage in mice. Mice were orally administrated with or without PLE of single dose (250 mg/kg/day) for 21 days. Mouse skin was directly exposed to UVB irradiation from the vertical position to its back at a dose of 200 mJ/cm2. In vitro study using HaCaT keratinocytes, UVB decreased cell viability and increased the production of an inflammatory marker. PLE recovered the cell viability and suppressed the production of Th2 chemokines (CCL17 and CCL27). Moreover, oral administration of PLE to UVB-induced mice led to a striking suppression of the skin demage. Histologic analyses revealed that infiltration of inflammatory cells, especially degranulated mast cells, thickening of the epidermis and hyperplasia were significantly reduced. The proliferation levels of production of draining spleen cells were markedly suppressed by the treatment with PLE. Furthermore, UVB-induced ex vivo CCL17 and CCL27 production of draining spleen cells was markedly diminished by PLE oral administration. PLE treatment, also suppressed UVB-induced CCL17 and CCL27 expressions in skin. These results suggest that PLE has a potential use as a compound for protection against UVB-induced skin inflammation.
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KEYWORD
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UVB, Persimmon Leaf extract, Protective effect
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